Compound as reactive (1)

6-aminopenicillanate + phenylacetyl-CoA → coenzyme A + penicillin G + H⁺

Compound as product (1)

H₂O + isopenicillin N → 6-aminopenicillanate + L-2-aminoadipate

Reversible reaction (1)

H₂O + penicillin G ↔ 6-aminopenicillanate + phenylacetate