Compound as reactive (1)

coenzyme A + 3-hydroxypimeloyl-CoA → 2 H⁺ + glutaryl-CoA + acetyl-CoA

Compound as product (2)

H₂O + 6-carboxyhex-2-enoyl-CoA → 3-hydroxypimeloyl-CoA

H₂O + pimeloyl-CoA → 3-hydroxypimeloyl-CoA

Reversible reaction (3)

2 H₂O + 6-oxocyclohex-1-ene-1-carbonyl-CoA ↔ 3-hydroxypimeloyl-CoA + H⁺

3-hydroxypimeloyl-CoA + NAD⁺ ↔ 3-oxopimeloyl-CoA + NADH + H⁺

H₂O + 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA ↔ 3-hydroxypimeloyl-CoA + H⁺