Compound as reactive (1)

(S)-3-methyl-2-oxopentanoate + H⁺ → 2-methylbutanal + CO₂

Compound as product (3)

H₂O + NADP⁺ + L-isoleucine → ammonia + NADPH + (S)-3-methyl-2-oxopentanoate

(R)-2,3-dihydroxy-3-methylpentanoate → H₂O + (S)-3-methyl-2-oxopentanoate

H₂O + NAD⁺ + L-isoleucine → NADH + (S)-3-methyl-2-oxopentanoate + H⁺ + ammonium

Reversible reaction (4)

2-oxoglutarate + L-isoleucine ↔ (S)-3-methyl-2-oxopentanoate + L-glutamate

coenzyme A + (S)-3-methyl-2-oxopentanoate + NAD⁺ ↔ NADH + 2-methylbutanoyl-CoA + CO₂

2 an oxidized ferredoxin [iron-sulfur] cluster + coenzyme A + (S)-3-methyl-2-oxopentanoate ↔ 2 a reduced ferredoxin [iron-sulfur] cluster + H⁺ + 2-methylbutanoyl-CoA + CO₂

(S)-3-methyl-2-oxopentanoate + L-valine ↔ 3-methyl-2-oxobutanoate + L-isoleucine