Compound as reactive (2)

2 S-adenosyl-L-methionine + a reduced electron donor + N-acetyldemethylphosphinothricin tripeptide → 2 H⁺ + an oxidized electron donor + S-adenosyl-L-homocysteine + N-acetylphosphinothricin tripeptide + 5'-deoxyadenosine + L-methionine

methylcob(III)alamin + N-acetyldemethylphosphinothricin tripeptide → N-acetylphosphinothricin tripeptide + cob(II)alamin

Compound as product (1)

2 L-alanine + N-acetyldemethyl-L-phosphinothricin → 2 H₂O + N-acetyldemethylphosphinothricin tripeptide

Reversible reaction (1)

S-adenosyl-L-methionine + methylcob(III)alamin + N-acetyldemethylphosphinothricin tripeptide ↔ N-acetylphosphinothricin tripeptide + 5'-deoxyadenosine + L-methionine + cob(III)alamin