pMK-LPTG-K

Ancestors

All plasmids

pMK-GFP

pMK-LPTG-K

Main features

aroL-ppsA-tktA-aroG^fbr, psiK_Pc

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*aroL*	Escherichia coli	Overexpression
*ppsA*	Escherichia coli	Overexpression
*tktA*	Escherichia coli	Overexpression
*aroG*	Escherichia coli	Feedback insensitive
*psiK*	Psilocybe cubensis	Overexpression

Strains harboring plasmid pMK-LPTG-K

Escherichia coli PP26

References

Guo, Cui; Luu, Nguyen N.T.; Adwer, Maryem M.; Hosseinzadeh, Hemen; Balan, Venkatesh; Yan, Yajun & Lin, Yuheng. Engineering Non-Natural Biosynthetic Pathways for Efficient Microbial Production of Psilocybin and Psilocin. Metabolic Engineering. 2025.