pRSFDuet-1

Ancestors

All plasmids

pRSFDuet-1

Derivated

Main features

RSF1030 ori, lacI, Two P_T7lac

Selection marker:

Kanamycin

References

Ming-Yue Fang, Chong Zhang, Song Yang, Jin-Yu Cui, Pei-Xia Jiang, Kai Lou, Masaaki Wachi & Xin-Hui Xing (2015). High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microbial Cell Factories.
Wenjuan Zha, Sheryl B. Rubin-Pitel, Zengyi Shao & Huimin Zhao (2009). Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering. Metabolic Engineering.
Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.
Ke, Qin; Liu, Chang; Zhuang, Yibin; Xue, Yaju; Cui, Zhanzhao; Zhang, Cuiying; Yin, Hua & Liu, Tao. Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose. Microbial Cell Factories. 2024, 23(1).
Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.
Yang, Haiquan; Xue, Yuxiang; Yang, Cui; Shen, Wei; Fan, You & Chen, Xianzhong. Modular Engineering of Tyrosol Production in Escherichia coli. Journal of Agricultural and Food Chemistry. 2019, 67(14), 3900-3908.
Hou, Yanan; Liu, Xue; Li, Shilin; Zhang, Xue; Yu, Sili & Zhao, Guang-Rong. Metabolic Engineering of Escherichia coli for de Novo Production of Betaxanthins. Journal of Agricultural and Food Chemistry. 2020, 68(31), 8370-8380.
Liu, Tongle; Zhang, Kang; Zhang, Mengwei; Wang, Luyao; Wu, Yi; Cai, Bohan; Gao, Shengqi; Wu, Mengping; Wu, Wentai; Wu, Jing & Su, Lingqia. De Novo Synthesis of Lacto-N-Neotetraose in Escherichia coli through Metabolic Engineering with Glucose as the Sole Carbon Source. Journal of Agricultural and Food Chemistry. 2025, 73(22), 13736-13745.