pCA24N

Ancestors

Derivated

• pKH15
• pKH19
• pCysH
• pCysI
• pCysJ
• pCysK
• pCysM
• pGrx1
• pGrx2
• pGrx3
• pGrx4
• pTrx1
• pTrx2
• pNrdH
• pCysK-I
• pCysK-J
• pCysJ-I
• pCysK-I-NrdH
• pOmpA
• pOmpC
• pOmpF
• pOmpT
• pOmpX
• pTolC
• pDsbA
• pGlpE
• pYceA
• pSseA
• pYibN
• pTusA
• pTusD

Main features

P_T5-lac

Selection marker:

Chloramphenicol

Strains harboring plasmid pCA24N

• Escherichia coli ΔcysK/pDES/pCA24N
• Escherichia coli ΔcysM/pDES/pCA24N
• Escherichia coli ΔcysM/pCA24N
• Escherichia coli WT/pDES/pCA24N

References

• Mireille Ginesy, Jaroslav Belotserkovsky, Josefine Enman, Leif Isaksson & Ulrika Rova (2015). Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis. Microbial Cell Factories.
• Natthawut Wiriyathanawudhiwong, Iwao Ohtsu, Zhao-Di Li, Hirotada Mori & Hiroshi Takagi (2008). The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. Applied Microbiology and Biotechnology.
• Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.