pGLY-2

Ancestors

All plasmids

pHHD01K

pGLY-2

Main features

P_tet-ycdW-aceA-aceK

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*ghrA*	Escherichia coli	Overexpression
*aceA*	Escherichia coli	Overexpression
*aceK*	Escherichia coli	Overexpression
*icd*	Escherichia coli	Attenuation

Strains harboring plasmid pGLY-2

Escherichia coli Mgly12P

References

Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.