pZPW74

Ancestors

All plasmids

pTrc99A

pZPW72

pZPW74

Derivated

Main features

hemA^C75A/R365K, P_J23100-coaA, P_ppc-ppc, P_eamA-eamA

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*hemA*	Rhodopseudomonas palustris	Feedback insensitive
*coaA*	Escherichia coli	Overexpression
*ppc*	Escherichia coli	Overexpression
*eamA*	Escherichia coli	Overexpression

Strains harboring plasmid pZPW74

Escherichia coli ALA13

References

Pu, W., Chen, J., Zhou, Y. et al. Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid. Biotechnol Biofuels 16, 31 (2023).