pS4

Ancestors

All plasmids

pBbB5c

pS4

Main features

pBBR1 ori, aroE-aroD-aroB^op-P_LtetO-1-aroG*-ppsA-tktA

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*aroE*	Escherichia coli	Overexpression
*aroD*	Escherichia coli	Overexpression
*aroB*	Escherichia coli	Overexpression
*aroG*	Escherichia coli	Feedback insensitive
*ppsA*	Escherichia coli	Overexpression
*tktA*	Escherichia coli	Overexpression

Strains harboring plasmid pS4

References

Darmawi Juminaga, Edward E. K. Baidoo, Alyssa M. Redding-Johanson, Tanveer S. Batth, Helcio Burd, Aindrila Mukhopadhyay, Christopher J. Petzold & Jay D. Keasling (2011). Modular Engineering of l-Tyrosine Production in Escherichia coli. Applied and Environmental Microbiology.