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pY3

Ancestors
  • All plasmids
    • pBbA5a
      • pY3



Main features
p15A ori, lacI,Plac-UV5-tyrB-tyrA*-aroC T1-Ptrc-aroA-aroL

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
tyrB
Escherichia coli
Overexpression
tyrA
Escherichia coli
Feedback insensitive
aroC
Escherichia coli
Overexpression
aroA
Escherichia coli
Overexpression
aroL
Escherichia coli
Overexpression



Strains harboring plasmid pY3
  • Escherichia coli F (pS4, pY3)
  • Escherichia coli G (pS5, pY3)

References
  • Darmawi Juminaga, Edward E. K. Baidoo, Alyssa M. Redding-Johanson, Tanveer S. Batth, Helcio Burd, Aindrila Mukhopadhyay, Christopher J. Petzold & Jay D. Keasling (2011). Modular Engineering of l-Tyrosine Production in Escherichia coli. Applied and Environmental Microbiology.

Reference

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