p15Trc-zgp

Ancestors

All plasmids

p15Trc

p15Trc-zg

p15Trc-zgp

Main features

p15A replication, P_trc-synRBS-zwf-synRBS-gnd-synRBS-pgl, zwf and gnd genes from C. glutamicum, pgl gene from E. coli

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*zwf*	Corynebacterium glutamicum	Feedback insensitive
*gnd*	Corynebacterium glutamicum	Feedback insensitive
*pgl*	Escherichia coli	Overexpression

Strains harboring plasmid p15Trc-zgp

Escherichia coli RF01S/p15Trc-zgp

References

Lin, Zhenquan; Xu, Zhibo; Li, Yifan; Wang, Zhiwen; Chen, Tao & Zhao, Xueming. Metabolic engineering of Escherichia coli for the production of riboflavin. Microbial Cell Factories. 2014, 13(1).