pETDuet-ldhL-fdh

Ancestors

All plasmids

pETDuet-1

pETDuet-fdh

pETDuet-ldhL-fdh

Main features

ldhL of B. coagulans and fdh of Candida boidinii

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*ldhL*	Bacillus coagulans	Overexpression
*fdh*	Candida boidinii	Overexpression

Strains harboring plasmid pETDuet-ldhL-fdh

Escherichia coli BL21(pETDuet-ldhL-fdh)

References

Zheng, Zhaojuan; Zhao, Mingyue; Zang, Ying; Zhou, Ying & Ouyang, Jia. Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase. Journal of Biotechnology. 2015, 207, 47-51.