pSC101trp

Ancestors

All plasmids

pSC101trp

Main features

pSC101 ori, tac_pro-aroH^fbr, tac_pro-trpE^fbrDCBA

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*aroH*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive
*trpD*	Escherichia coli	Overexpression
*trpC*	Escherichia coli	Overexpression
*trpB*	Escherichia coli	Overexpression
*trpA*	Escherichia coli	Overexpression

Strains harboring plasmid pSC101trp

Escherichia coli HTP101-LMT

References

Xu, Da; Fang, Mengjun; Wang, Haijiao; Huang, Lei; Xu, Qinyang & Xu, Zhinan. Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway. Applied Microbiology and Biotechnology. 2020, 104(6), 2481-2488.