pCDFD-rtxA-D9

Ancestors

All plasmids

pCDFDuet-1

pCDFD-rtxA-D9

Main features

CloDF13 ori, P_T7-rtxA (residues 1-345 and 463 pb upstream of rtxA)

Selection marker:

Streptomycin

Spectinomycin

Modifications

Gene	Organism	Modification
*rtxA*	Bradyrhizobium elkanii	Overexpression

Strains harboring plasmid pCDFD-rtxA-D9

Escherichia coli HMS174(DE3)/pCDFD-rtxA-D9

References

Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.