pQE1a-mMsB/EcA

Ancestors

All plasmids

pQE1a-Red

pQE1a-MsB

pQE1a-mMsB

pQE1a-mMsB/EcA

Main features

ColE1 ori, P_tac-MBP-egtB and gshA

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*egtB*	Mycobacterium smegmatis	Overexpression
*gshA*	Escherichia coli	Overexpression

Strains harboring plasmid pQE1a-mMsB/EcA

Escherichia coli ET4

References

Ryo Osawa, Tomoyuki Kamide, Yasuharu Satoh, Yusuke Kawano, Iwao Ohtsu & Tohru Dairi (2018). Heterologous and High Production of Ergothioneine in Escherichia coli. Journal of Agricultural & Food Chemistry.