pDG33

Ancestors

All plasmids

pET-28a(+)

pDG33

Main features

P_T7: aroG^fbr and trpE^fbr

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive

References

Daoyi Guo, Sijia Kong, Xu Chu, Xun Li & Hong Pan (2019). De Novo Biosynthesis of Indole-3-acetic Acid in Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.