Ancestors

Main features

alaD_Bs

Selection marker:

Ampicillin

Strains harboring plasmid pTrc99A-alaD

• Escherichia coli ALS929 pTrc99A-alaD
• Escherichia coli ALS887 pTrc99A-alaD

References

• Smith, Geoffrey M.; Lee, Sarah A.; Reilly, Kevin C.; Eiteman, Mark A. & Altman, Elliot. Fed-batch two-phase production of alanine by a metabolically engineered Escherichia coli. Biotechnology Letters. 2006, 28(20), 1695-1700.
• M. Lee, G. M. Smith, M. A. Eiteman & E. Altman (2004). Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene. Applied Microbiology and Biotechnology.