placI-rib

Ancestors

All plasmids

pACYC184

pACYCDuet-1

placI-rib

Main features

lacI, P_tac-ribEDAH

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*ribE*	Bacillus subtilis	Overexpression
*ribD*	Bacillus subtilis	Overexpression
*ribA*	Bacillus subtilis	Overexpression
*ribH*	Bacillus subtilis	Overexpression
*lacI*	Escherichia coli	Overexpression

Strains harboring plasmid placI-rib

Escherichia coli EF-18

References

Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.