pETDuet-ais-tbrB

Ancestors

All plasmids

pETDuet-1

pETDuet-ais-tbrB

Main features

Ais and TbrB

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*ais*	Pseudomonas sp. WU-0701	Overexpression
*tbrB*	Bacillus thuringiensis serovar chinensis CT-43	Overexpression

Strains harboring plasmid pETDuet-ais-tbrB

Escherichia coli W3110-TAA01

References

Leilei Guo, Yi Cheng, Xiaoxu Tan, Hongxu Zhang, Hairong Yu, Wenjia Tian, Mingyuan Liu, Weikang Sun, Kaiyu Gao, Tianyi Jiang, Qianjin Kang, Wensi Meng, Yidong Liu, Zhaoqi Kang, Chuanjuan Lü, Chao Gao, Ping Xu & Cuiqing Ma (2025). Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli. Synthetic and Systems Biotechnology.