pDtac15-At2tUG

Ancestors

All plasmids

pTac15K

pDtac15-At2tUG

Main features

P_tac-act_Cp-acl2_Cp, P_tac-acuI_Ec-gctAB_Af

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*act*	Clostridium propionicum	Overexpression
*acl2*	Clostridium propionicum	Overexpression
*yhdH*	Escherichia coli	Overexpression
*gctA*	Acetobacter fermentans	Overexpression
*gctB*	Acetobacter fermentans	Overexpression

Strains harboring plasmid pDtac15-At2tUG

Escherichia coli EPA1+pDtac15-At2tUG

References

Ahn, Da-Hee; Ko, Yoo-Sung; Prabowo, Cindy Pricilia Surya & Lee, Sang Yup. Microbial production of propionic acid through a novel β-alanine route. Metabolic Engineering. 2026, 93, 219-231.