Substrate yield in Fed-Batch for glycolate

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
E. coli Mgly13
E. coli Mgly134
E. coli EYX-2
E. coli Mgly434

Substrate yield in Flask for glycolate

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
E. coli BW25113-pGLY-1
E. coli EXY-1-pGLY-1
E. coli Mgly12
E. coli Mgly11
E. coli Mgly12P
E. coli Mgly13
E. coli EYX-2
E. coli Mgly134
E. coli EYX-3
E. coli Mgly534
E. coli Mgly334
E. coli Mgly434

Substrate yield in Batch for glycolate

Strain Production
g/g of sustrate
0.1
0.2
0.3
0.4
0.5
E. coli EYX-2

Titer in Flask for glycolate

Strain Production
mg/L
500.0
1000.0
1500.0
2000.0
2500.0
3000.0
E. coli BW25113-pGLY-1
E. coli EXY-1-pGLY-1
E. coli Mgly12
E. coli Mgly11
E. coli Mgly13
E. coli Mgly134
E. coli Mgly534
E. coli Mgly12P
E. coli Mgly334
E. coli EYX-1-glcb-1-pGLY-1
E. coli EYX-2
E. coli Mgly434

Titer in Fed-Batch for glycolate

Strain Production
mg/L
10000
20000
30000
40000
50000
60000
70000
E. coli EYX-2
E. coli Mgly434

Titer in Batch for glycolate

Strain Production
mg/L
1000
2000
3000
4000
5000
6000
7000
8000
9000
E. coli EYX-2