Escherichia coli ALA13

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pZCA136 added | Plasmid pZPW74 added

Bars (|) indicate differences between strains.


Plasmids

  • pZCA136 (sRsucC, sRhemB, sRaceA, Ptet, T1/TE)

    pZPW74 (hemAC75A/R365K, PJ23100-coaA, Pppc-ppc, PeamA-eamA)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
5-aminolevulinate Titer 5.69 g/L 6.739 OD600* Flask [239]
5-aminolevulinate Substrate yield 0.495 mol/mol of substrate 6.739 OD600* Flask [239]
5-aminolevulinate Titer 6.857 g/L * 7.674 OD600* Flask [239]
5-aminolevulinate Substrate yield 0.45 mol/mol of substrate * 7.674 OD600* Flask [239]
5-aminolevulinate Titer 8.008 g/L * 6.049 OD600* Flask [239]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References