Escherichia coli ALA14

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pZCA136 added | Plasmid pZPW76 added

Bars (|) indicate differences between strains.


Plasmids

  • pZCA136 (sRsucC, sRhemB, sRaceA, Ptet, T1/TE)

    pZPW76 (hemAC75A/R365K, PJ23100-coaA, Pppc-ppc, PeamA-eamA, PgrxA-grxA)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
5-aminolevulinate Titer 8.32 g/L 8.372 OD600* Flask [239]
5-aminolevulinate Substrate yield 0.559 mol/mol of substrate * 8.372 OD600* Flask [239]
5-aminolevulinate Titer 9.79 g/L * 6.968 OD600* Flask [239]
5-aminolevulinate Titer 4.5 g/L * 44.84 OD600* Fed-Batch [239]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References