Escherichia coli ALA26

Ancestors

Genotype with respect to parental

ΔcoaA


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔcoaA | Plasmid pZCA136 added | Plasmid pZPW76 added

Bars (|) indicate differences between strains.


Plasmids

  • pZCA136 (sRsucC, sRhemB, sRaceA, Ptet, T1/TE)

    pZPW76 (hemAC75A/R365K, PJ23100-coaA, Pppc-ppc, PeamA-eamA, PgrxA-grxA)


Modifications
Gene Organism Modification
Silencing

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
5-aminolevulinate Titer 30.7 g/L 31.9 OD600* 32 h * Fed-Batch [239]
5-aminolevulinate Substrate yield 0.532 mol/mol of substrate 31.9 OD600* 32 h * Fed-Batch [239]
5-aminolevulinate Volumetric productivity 1.02 g/L/h 31.9 OD600* 32 h * Fed-Batch [239]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References