Escherichia coli B2/pED + pVio

Ancestors

Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔtrpR | Plasmid pVio added | Plasmid pED added

Bars (|) indicate differences between strains.


Plasmids

  • pED (PT7-trpEfbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG)-trpD)

    pVio (vioABCDE)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
violacein Titer 0.603 g/L 1.5898 OD600* 48 h Flask [96]
violacein Titer 1.75 g/L Fed-Batch [96]
violacein Volumetric productivity 36.0 mg/L/h Fed-Batch [96]
violacein Substrate yield 0.116 g/g of sustrate Flask [96]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References