Escherichia coli RF03T

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli RF02

Escherichia coli RF03

Escherichia coli RF03T

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Δedd, Δeda | Plasmid pLS01 added

Bars (|) indicate differences between strains.

Plasmids

pLS01 (pSC101 replicon, P_trc ΔlacI^q ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
riboflavin	Titer	516.0 mg/L		Glucose	23 h	Flask	[247]
riboflavin	Titer	502.0 mg/L	4.0 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	50.2 mg/g of sustrate	4.0 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.5 mg/g DCW/h	4.0 g/L	Glucose		Flask	[247]
riboflavin	Titer	540.0 mg/L	4.1 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	54.1 mg/g of sustrate	4.1 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.7 mg/g DCW/h	4.1 g/L	Glucose		Flask	[247]
riboflavin	Titer	570.0 mg/L	4.2 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	57.2 mg/g of sustrate	4.2 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.9 mg/g DCW/h	4.2 g/L	Glucose		Flask	[247]
riboflavin	Titer	597.0 mg/L	4.5 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	59.6 mg/g of sustrate	4.5 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.8 mg/g DCW/h	4.5 g/L	Glucose		Flask	[247]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.