Escherichia coli RF05S-M40

Ancestors

Genotype with respect to parental

Ptrc-acs, ribF-RBS*


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Δedd, Δeda | Ptrc-acs, ribF-RBS* | Plasmid p20C-EC10 added

Bars (|) indicate differences between strains.


Plasmids

  • p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)


Modifications
Gene Organism Modification
acs
Overexpression
Attenuation

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 2702.8 mg/L Flask [247,248]
riboflavin Substrate yield 137.5 mg/g of sustrate Flask [247]
riboflavin Titer 1036.1 mg/L Flask [248]
riboflavin Titer 1507.0 mg/L Flask [248]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References