Escherichia coli RF01S/p15Trc-zg

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid p20C-EC10 added | Plasmid p15Trc-zg added

Bars (|) indicate differences between strains.


Plasmids

  • p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

    p15Trc-zg (p15A replication, Ptrc-synRBS-zwf-synRBS-gnd, zwf and gnd genes from C. glutamicum)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 197.4 mg/L 3.0 OD600 Flask [248]
riboflavin Substrate yield 24.2 mg/g of sustrate 3.0 OD600 Flask [248]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References