Escherichia coli ET11

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pTrc-ectABC added | ΔlysA | ΔpykF | Plasmid pTrc-ectABC lost | Plasmid pTETB added

Bars (|) indicate differences between strains.


Plasmids

  • pTETB (Ptrc-ectABCHv-Ptrc-ectBHv)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
ectoine Titer 12.9 g/L 17.54 g/L* 48 h * Flask [253]
ectoine Titer 53.2 g/L 26.9 g/L* 48 h * Fed-Batch [253]
ectoine Biomass-specific yield 0.74 g/g DCW 17.54 g/L* 48 h * Flask [253]
ectoine Volumetric productivity 1.11 g/L/h 26.9 g/L* 48 h Fed-Batch [253]
ectoine Substrate yield 0.33 g/g of sustrate 26.9 g/L* 48 h Fed-Batch [253]
ectoine Biomass-specific yield 2.0 g/g DCW 26.9 g/L* 48 h Fed-Batch [253]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References