Escherichia coli ET13

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli ET01

Escherichia coli ET03

Escherichia coli ET08

Escherichia coli ET08-N

Escherichia coli ET13

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pTrc-ectABC added | ΔlysA | ΔpykF | Plasmid pTrc-ectABC lost | Plasmid pTETC added

Bars (|) indicate differences between strains.

Plasmids

pTETC (P_trc-ectABC_Hv-P_trc-ectC_Hv)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
ectoine	Titer	9.05 g/L	14.58 g/L^*	Glucose	48 h ^*	Flask	[253]
ectoine	Biomass-specific yield	0.62 g/g DCW	14.58 g/L^*	Glucose	48 h ^*	Flask	[253]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li & Hong Xu (2022). Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor. Frontiers in Bioengineering and Biotechnology.