Escherichia coli HS27
Ancestors
Genotype with respect to parental
Ptrc::Pppc
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔJIB* ΔthrB ΔmetA ΔlysA ΔiclR ΔptsG ΔgalR ΔlacI::Ptrc-rhtA Ptrc-rhtA Ptrc-eamA Ptrc-metL Ptrc-thrA Ptrc-glk Ptrc-gltB | Δadd Δamn | Ptrc-ado1Sc | Δyjit::Ptrc-thrAfbr | Ptrc::Pasd | ΔgapC::Ptrc-aspC | ΔgapC::Ptrc-aspC | Ptrc::PppcBars (|) indicate differences between strains.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-homoserine | Titer | 119.96 g/L | Fed-Batch | [255] | |||
| L-homoserine | Substrate yield | 0.41 g/g of sustrate | Fed-Batch | [255] | |||
| L-homoserine | Volumetric productivity | 1.31 g/L/h | Fed-Batch | [255] | |||
| L-homoserine | Titer | 32.55 g/L | 11.19 OD600* | Flask | [255] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.