Escherichia coli Y3-4

Ancestors

Derived strains

Genotype with respect to parental

ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
add	Escherichia coli	Overexpression
adeD	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
hypoxanthine	Titer	1.67 g/L	46.0 OD₆₀₀^*	Glucose	Flask	[256]
AMP	Titer	0.11 g/L	46.0 OD₆₀₀^*	Glucose	Flask	[256]
xanthine	Titer	0.23 g/L ^*	46.0 OD₆₀₀^*	Glucose	Flask	[256]
guanine	Titer	0.15 g/L ^*	46.0 OD₆₀₀^*	Glucose	Flask	[256]
hypoxanthine	Substrate yield	0.035 g/g of sustrate ^*	46.0 OD₆₀₀^*	Glucose	Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.