Escherichia coli Y5-4A

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR | yghe::P_esaS-guaB | ycdn:: P_{BBa_j 23114}esaI

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
esaI	Pantoea stewartii	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
hypoxanthine	Titer	2.67 g/L	43.0 OD₆₀₀	Glucose	Flask	[256]
xanthine	Titer	0.1 g/L ^*	43.0 OD₆₀₀	Glucose	Flask	[256]
guanine	Titer	0.0 g/L ^*	43.0 OD₆₀₀	Glucose	Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.