Escherichia coli Y6-1

Ancestors

Derived strains

Escherichia coli Y6-2

Genotype with respect to parental

yeel::P_trc- glnA_Bs^{L159I, E304A}

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR | yghe::P_esaS-guaB | ycdn:: P_{BBa_j 23110}esaI | yjip::P_trc-pur(FDNTSQLMKECB) | yeel::P_trc- glnA_Bs^{L159I, E304A}

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
glnA	Bacillus subtilis	Feedback insensitive

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
hypoxanthine	Titer	3.61 g/L	45.0 OD₆₀₀^*	Glucose	24 h	Flask	[256]
hypoxanthine	Substrate yield	0.071 g/g of sustrate ^*	45.0 OD₆₀₀^*	Glucose	24 h	Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.