Escherichia coli TS-1A

Ancestors

Genotype with respect to parental

△ptsI

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △trpR, △tnaAB | Plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA added | △ptsI | Plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA lost | Plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A added

Bars (|) indicate differences between strains.

Plasmids

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A (P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A)

Modifications

Gene	Organism	Modification
ptsI	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	3.27 g/L ^*		Glucose		Flask	[258]
L-tryptophan	Substrate yield	0.086 g/g of sustrate ^*		Glucose		Flask	[258]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.