Escherichia coli ispANaFS-SN
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTispANaFS added | Plasmid pSSN12Didi addedBars (|) indicate differences between strains.
Plasmids
-
pSSN12Didi (mvaK1Sp, mvaK2Sp, mvaDSp, idiEc)
pTispANaFS (Ptrc-RBS-aFS-ispA fusion without linker)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (E,E)-α-farnesene | Titer | 62.2 mg/L | 10.4 OD600* | 48 h * | Flask | [261] | |
| (2E,6E)-farnesol | Titer | 22.3 mg/L * | 10.4 mg/L* | 48 h * | Flask | [261] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.