Escherichia coli ispALaFS-SN
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTispALaFS added | Plasmid pSSN12Didi addedBars (|) indicate differences between strains.
Plasmids
-
pSSN12Didi (mvaK1Sp, mvaK2Sp, mvaDSp, idiEc)
pTispALaFS (Ptrc-RBS-ispA-linker (GGGGS)-aFS)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (E,E)-α-farnesene | Titer | 86.8 mg/L | 14.63 OD600* | 48 h | Flask | [261] | |
| (2E,6E)-farnesol | Titer | 0.0 mg/L | 14.63 OD600* | 48 h | Flask | [261] | |
| (E,E)-α-farnesene | Titer | 153.6 mg/L | 19.26 OD600* | Flask | [261] | ||
| (E,E)-α-farnesene | Titer | 9.8 mg/L | Flask | [261] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.