Escherichia coli ispALaFS-NA

Ancestors

Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTispALaFS added | Plasmid pSNA added

Bars (|) indicate differences between strains.


Plasmids

  • pTispALaFS (Ptrc-RBS-ispA-linker (GGGGS)-aFS)

    pSNA (mvaEEf, mvaSEf, mvaK1Sp, mvaK2Sp, mvaDSp and idiEc)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
(E,E)-α-farnesene Titer 380.0 mg/L 21.1 OD600* 48 h * Flask [261]
(R)-mevalonate Titer 16.83 mg/L * 21.1 OD600* 48 h * Flask [261]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References