Escherichia coli VHY13

Ancestors

Derived strains

Genotype with respect to parental

yeeP::P_trc-spoT^{[R290E, K292D]}

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, ycdN::P_trc-alsS_Bs | ydeU::P_trc-alsS | yjiV::P_trc-ilvED | ycgH::P_trc-ilvC | yjiT::P_trc-brnFE | yeeP::P_trc-spoT^{[R290E, K292D]}

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
yeeP	Escherichia coli	Silencing
spoT	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-valine	Titer	39.1 g/L	43.0 OD₆₀₀^*	Glucose		Flask	[263]
L-valine	Substrate yield	0.32 g/g of sustrate ^*	43.0 OD₆₀₀^*	Glucose		Flask	[263]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yanan Hao, Qian Ma, Xiaoqian Liu, Xiaoguang Fan, Jiaxuan Men, Heyun Wu, Shuai Jiang, Daoguang Tian, Bo Xiong & Xixian Xie (2020). High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation. Metabolic Engineering.