Escherichia coli VHY18

Ancestors

Genotype with respect to parental

P_trc-ilvE::P_trc-bcd, ilvE::P_trc-bcd

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, ycdN::P_trc-alsS_Bs | ydeU::P_trc-alsS | yjiV::P_trc-ilvED | ycgH::P_trc-ilvC | yjiT::P_trc-brnFE | yeeP::P_trc-spoT^{[R290E, K292D]} | ΔldhA, ΔpflB, ΔadhE | P_trc-ilvC::P_trc-ilvC^M, ilvC::P_trc-ilvC^M | P_trc-ilvE::P_trc-bcd, ilvE::P_trc-bcd

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
ilvE	Escherichia coli	Silencing
bcd	Bacillus subtilis	Overexpression
ilvE	Escherichia coli	Silencing
bcd	Bacillus subtilis	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Scale	Ref.
L-valine	Titer	84.0 g/L	Glucose	Fed-Batch	[263]
L-valine	Substrate yield	0.41 g/g of sustrate	Glucose	Fed-Batch	[263]
L-valine	Volumetric productivity	2.33 g/L/h	Glucose	Fed-Batch	[263]
L-valine	Titer	86.0 g/L	Glucose	Fed-Batch	[263]
L-valine	Substrate yield	0.33 g/g of sustrate	Glucose	Fed-Batch	[263]
L-valine	Volumetric productivity	1.95 g/L/h	Glucose	Fed-Batch	[263]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yanan Hao, Qian Ma, Xiaoqian Liu, Xiaoguang Fan, Jiaxuan Men, Heyun Wu, Shuai Jiang, Daoguang Tian, Bo Xiong & Xixian Xie (2020). High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation. Metabolic Engineering.