Escherichia coli LEU28
Ancestors
Genotype with respect to parental
trpR::Pbs3-esaI
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ycjV::Ptrc-leuAfbrEc | yciQ::Ptrc-leuAfbrEc | yghE::Ptrc-leuAfbrEc | yjgX::Ptrc-leuBCD | yjiT::Ptrc-ilvIHfbr | ylbE::Ptrc-ilvIHfbr | yjiV::Ptrc-ilvEDC | PleuA::Ptrc | ΔlivK | livJ::Ptrc-azlC | brnQ::Ptrc-brnFE | yjiP::pntAB | ilvE::Ptrc-Bcldh | ΔpoxB | ΔpflB | ΔldhA | yeeP::PapFAB104-esaR | PsucAB::PesaS | trpR::Pbs3-esaIBars (|) indicate differences between strains.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-leucine | Titer | 12.66 g/L * | 34.74 OD600* | Flask | [265] | ||
| L-leucine | Substrate yield | 0.16 g/g of sustrate * | 34.74 OD600* | Flask | [265] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.