Escherichia coli LJL12
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlysA | ΔmetA | ΔthrBC | ΔiclR | ΔgltA | ΔpykA | ΔpykF | Plasmid pKK-miniPtac-thrA addedBars (|) indicate differences between strains.
Plasmids
-
pKK-miniPtac-thrA (miniPtac-thrA)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-homoserine | Titer | 3.2 g/L | 2.8 OD600 | 44 h | Flask | [271] | |
| L-homoserine | Titer | 35.8 g/L | 22.0 OD600 | 40 h | Fed-Batch | [271] | |
| L-homoserine | Volumetric productivity | 0.82 g/L/h | 22.0 OD600 | 40 h | Fed-Batch | [271] | |
| L-homoserine | Substrate yield | 0.35 g/g of sustrate | 22.0 OD600 | 40 h | Flask | [271] | |
| acetate | Titer | 6.9 g/L | 22.0 OD600 | 40 h | Fed-Batch | [271] | |
| acetate | Substrate yield | 0.069 g/g of sustrate | 22.0 OD600 | 40 h | Fed-Batch | [271] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.