Escherichia coli HTP101-LMT

Ancestors

Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔtnaA | Plasmid pSC101trp added | Plasmid pACHTP-LMT added

Bars (|) indicate differences between strains.


Plasmids

  • pACHTP-LMT (p15A ori, Lacpro-mtrA-PTPS-SPR, M1-93pro-PCD-DHPR, T7pro-TPH145)

    pSC101trp (pSC101 ori, tacpro-aroHfbr, tacpro-trpEfbrDCBA)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 1.54 g/L 15.97 OD600 90 h Flask [273]
5-hydroxy-L-tryptophan Titer 1.21 g/L 15.97 OD600 90 h Flask [273]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References