Escherichia coli TRP30

Ancestors

Derived strains

• Escherichia coli TRP30/pSCHTP-LMT

Genotype with respect to parental

Replace the tac_pro of aroH^fbr with the M1-30_pro

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔtnaA | ΔaroH | ΔaroH:tac_pro-aroH^fbr | ΔtrpE | ΔtrpE:tac_pro-trpE^fbr | Replace the tac_pro of aroH^fbr with the M1-30_pro

Bars (|) indicate differences between strains.

References

• Xu, Da; Fang, Mengjun; Wang, Haijiao; Huang, Lei; Xu, Qinyang & Xu, Zhinan. Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway. Applied Microbiology and Biotechnology. 2020, 104(6), 2481-2488.