Escherichia coli KW002
Ancestors
Derived strains
Genotype with respect to parental
ΔtrpR ΔtrpL
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔtrpR ΔtrpLBars (|) indicate differences between strains.
Plasmids
-
PH5a1 (aroGfbr trpEDCBA)
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Silencing
|
||
|
Overexpression
|
||
|
Overexpression
|
||
|
Overexpression
|
||
|
Overexpression
|
||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 1.0 g/L | Flask | [135] | |||
| L-tryptophan | Substrate yield | 0.081 g/g of sustrate | Flask | [135] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.