Escherichia coli TRP0

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli BL21ΔtnaA

Escherichia coli BL21ΔtnaAΔaroH

Escherichia coli BL21ΔtnaAΔaroH(aroH^fbr)

Escherichia coli BL21ΔtnaAΔaroH(aroH^fbr, ΔtrpE)

Escherichia coli TRP0

Derived strains

Genotype with respect to parental

ΔtrpE:tac_pro-trpE^fbr

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔtnaA | ΔaroH | ΔaroH:tac_pro-aroH^fbr | ΔtrpE | ΔtrpE:tac_pro-trpE^fbr

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
trpE	Escherichia coli	Feedback insensitive
trpD	Escherichia coli	Overexpression
trpC	Escherichia coli	Overexpression
trpB	Escherichia coli	Overexpression
trpA	Escherichia coli	Overexpression

References

Xu, Da; Fang, Mengjun; Wang, Haijiao; Huang, Lei; Xu, Qinyang & Xu, Zhinan. Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway. Applied Microbiology and Biotechnology. 2020, 104(6), 2481-2488.