Escherichia coli HTP11

Ancestors

Genotype with respect to parental

mbhA::PM1−30-gdhesi


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆tnaA, lacIZ::PxylF-T7RNAP, mlc::mlc* | yghX::Ptrc-mtrA-PTPS-SPR | yjgX::PT7-PCD-DHPR | Plasmid pSTV-TM2 added | trpLE::Ptrc-trpEfbr | yjiV::Ptrc-aroGfbr | ycgH::Ptrc-serAfbr | mbhA::PM1−30-gdhesi

Bars (|) indicate differences between strains.


Plasmids

Modifications
Gene Organism Modification
Silencing
gdh
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
5-hydroxy-L-tryptophan Titer 8.58 g/L 75.3 OD600 32 h Fed-Batch [274]
5-hydroxy-L-tryptophan Substrate yield 0.095 g/g of sustrate 75.3 OD600 32 h Fed-Batch [274]
5-hydroxy-L-tryptophan Volumetric productivity 0.48 g/L/h 75.3 OD600 32 h Fed-Batch [274]
5-hydroxy-L-tryptophan Titer 3.54 g/L 49.9 OD600* Flask [274]
5-hydroxy-L-tryptophan Substrate yield 0.088 g/g of sustrate 49.9 OD600* Flask [274]
L-tryptophan Titer 0.32 g/L 49.9 OD600* Flask [274]
L-tryptophan Titer 0.5 g/L 75.3 OD600 32 h Fed-Batch [274]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References