Escherichia coli S092/pScTrpC

Ancestors

Genotype with respect to parental

TrpC(S. cerevisiae)

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA | ΔtrpC | TrpC(S. cerevisiae) | Plasmid pScTrpC added

Bars (|) indicate differences between strains.

Plasmids

pScTrpC (pSC101 ori, P_tac, trp3_Sc trp1_Sc)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	3.13 g/L		Glucose	20 h	Batch	[165]
L-tryptophan	Substrate yield	0.11 g/g of sustrate		Glucose	20 h	Batch	[165]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.