Escherichia coli HMS174ΔserB::rtxA-D9 and serB

Ancestors

Genotype with respect to wild type

F⁺ (λ) | F⁻ recA1, IN(rrnD-rrnE)1, rph-1, rpoB331(RifR), hsdR19, λ(DE3[lacI, lacUV5-T7 gene 1, ind1, sam7, nin5]), (Rif^r ) | ΔserB | Plasmid pET-rtxA-D9 added | Plasmid pACYCD-serBeco added

Bars (|) indicate differences between strains.

Plasmids

• pET-rtxA-D9 (rtxA (residues 1-345 and 66 pb upstream of rtxA))

  pACYCD-serBeco (p15A ori, lacI P_T7lac, P_T7lac-serB)

References

• Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.